4AQS

Laminin beta1 LN-LE1-4 structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-18
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	143.990, 152.950, 92.985
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.475 - 3.109
R-factor	0.2462
Rwork	0.246
R-free	0.28710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2Y38 AND 1KLO
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.090	0.660
Number of reflections	18789
<I/σ(I)>	12.9	2.7
Completeness [%]	99.5	99.3
Redundancy	6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		pH 5.0