4AQQ
Dodecahedron formed of penton base protein from adenovirus Ad3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-07-04 |
| Detector | MARRESEARCH MAR133 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 342.701, 342.701, 342.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 242.330 - 4.750 |
| R-factor | 0.29104 |
| Rwork | 0.291 |
| R-free | 0.29029 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9g |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.300 | 5.010 |
| High resolution limit [Å] | 4.750 | 4.750 |
| Rmerge | 0.212 | 0.582 |
| Number of reflections | 67599 | |
| <I/σ(I)> | 3.3 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | HANGING DROP METHOD WITH 2UL:2UL DROPS. RESERVOIR 12 % PEG 8000, 100 MM HEPES PH 6.5, 100 MM CACL2 |
