4AQG
X-ray crystallographic structure of Crimean-congo haemorrhagic fever virus nucleoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-11 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.784, 64.503, 127.719 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.520 - 2.800 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.130 | 0.780 |
Number of reflections | 11246 | |
<I/σ(I)> | 17 | 1.4 |
Completeness [%] | 99.7 | 96.8 |
Redundancy | 12.9 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 12% (V/V) PEG 500, 0.1 M MES, PH 6.5 |