4AQA
CRYSTAL STRUCTURE OF DEAFNESS ASSOCIATED MUTANT MOUSE CADHERIN-23 EC1- 2D124G AND PROTOCADHERIN-15 EC1-2 FORM I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.293, 40.506, 84.629 |
| Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
| Resolution | 34.190 - 1.960 |
| R-factor | 0.18035 |
| Rwork | 0.178 |
| R-free | 0.22775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4apx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.327 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.190 | 2.000 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.110 | 0.400 |
| Number of reflections | 41729 | |
| <I/σ(I)> | 8.5 | 2.4 |
| Completeness [%] | 99.4 | 95.5 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 MES, 8% PEG 20000, PH 6.5 |
