4APX
CRYSTAL STRUCTURE OF MOUSE CADHERIN-23 EC1-2 AND PROTOCADHERIN-15 EC1- 2 FORM I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-04 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.606, 40.469, 84.623 |
| Unit cell angles | 90.00, 103.12, 90.00 |
Refinement procedure
| Resolution | 32.030 - 1.650 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.19307 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2WHV AND HOMOLOGY MODEL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.415 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.030 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 66852 | |
| <I/σ(I)> | 25.6 | 2.8 |
| Completeness [%] | 96.9 | 92.8 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M MES, 8% PEG8000, PH6.5 |
