4APS
Crystal structure of a POT family peptide transporter in an inward open conformation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2009-07-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.390, 112.990, 215.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.620 - 3.300 |
| R-factor | 0.2731 |
| Rwork | 0.272 |
| R-free | 0.28950 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.100 | 1.110 |
| Number of reflections | 33300 | |
| <I/σ(I)> | 5.1 | 1.1 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 26 % PEG 400, 0.1 M MES PH 6.50, 0.03 M MGCL2 0.001 M CDCL2 |
