4APM

Crystal Structure of AMA1 from Babesia divergens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	67.820, 139.180, 45.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.830 - 2.300
R-factor	0.19281
Rwork	0.190
R-free	0.25049
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q8a
RMSD bond length	0.010
RMSD bond angle	1.329
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.830	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.390
Number of reflections	19533
<I/σ(I)>	12.5	3.7
Completeness [%]	99.2	96.4
Redundancy	4.6	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				16% PEG 8000, 300 MM CALCIUM ACETATE, 100 MM SODIUM CACODYLATE PH 5.5 AND 3% 2-METHYL-2,4-PENTANEDIOL (MPD)