4ANR
Crystal structure of soluble lytic Transglycosylase SltB1 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.500, 91.500, 81.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.750 - 1.840 |
| R-factor | 0.18163 |
| Rwork | 0.178 |
| R-free | 0.21121 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qus |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.204 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.750 | 1.950 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 34561 | |
| <I/σ(I)> | 41.29 | 8.41 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 21 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.5 M MALONATE PH 6.5 AND 3 MM CACL2, 20MG/ML PROTEIN CONCENTRATION |
