4ANN
Crystal Structure Staphylococcus aureus ESSB cytoplasmic fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2009-05-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.704, 58.646, 95.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.050 |
| R-free | 0.18080 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.033 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.740 | 1.150 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.060 | 0.410 |
| Number of reflections | 100245 | |
| <I/σ(I)> | 18 | 3.6 |
| Completeness [%] | 99.0 | 92.8 |
| Redundancy | 7.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.18 M AMMONIUM CITRATE PH5.0, 20 % (W/V) PEG3350, 0.25 MM TRIS(2-CARBOXYETHYL)PHOSPHINEHYDROCHLORIDE, 5% GLYCEROL |
