4AN8
Structure of Thermus thermophilus CasA (Cse1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.360, 48.110, 129.780 |
| Unit cell angles | 90.00, 97.03, 90.00 |
Refinement procedure
| Resolution | 46.825 - 2.300 |
| R-factor | 0.1984 |
| Rwork | 0.196 |
| R-free | 0.24720 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.119 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.830 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 51896 | |
| <I/σ(I)> | 18.9 | 2.9 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2.1M NAAC, MES PH 6.5 |
