4AMT
Crystal structure at 2.6A of human prorenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2009-02-20 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 |
| Unit cell lengths | 141.320, 141.320, 75.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.330 - 2.600 |
| R-factor | 0.26626 |
| Rwork | 0.264 |
| R-free | 0.30024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v0z |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.690 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.077 | 0.718 |
| Number of reflections | 22788 | |
| <I/σ(I)> | 11.9 | 1.8 |
| Completeness [%] | 98.5 | 99.2 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.0 M LITHIUM SULFATE, 0.1 M CITRATE PH 5.6, 0.5 M AMMONIUM SULFATE |
