4AMK
Fab fragment of antiporphrin antibody 13g10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-08 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 55.800, 62.750, 113.500 |
| Unit cell angles | 90.00, 91.65, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.2244 |
| Rwork | 0.224 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1MFA AND 1MFE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.522 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.040 | 0.190 |
| Number of reflections | 23580 | |
| <I/σ(I)> | 23.7 | 4.6 |
| Completeness [%] | 93.7 | 91.4 |
| Redundancy | 2.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 26.5 % PEG 2000, 0.2 M MGCL2, 0.1 M TRIS PH 8.5, 10% GLYCEROL |
