4AMB
Crystal structure of the glycosyltransferase SnogD from Streptomyces nogalater
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.911, 170.955, 69.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.620 |
| R-factor | 0.22689 |
| Rwork | 0.223 |
| R-free | 0.29691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d0q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.497 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.760 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.140 | 0.460 |
| Number of reflections | 23310 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 97.6 | 86.7 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 26% (W/V) PEG 3350, 0.1 M CITRATE PH 5.2 |
