4AM4
Bacterioferritin from Blastochloris viridis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 170.650, 170.650, 170.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 98.520 - 1.680 |
| R-factor | 0.16595 |
| Rwork | 0.165 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gvy |
| RMSD bond length | 0.028 |
| RMSD bond angle | 3.049 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 1.720 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.070 | 0.690 |
| Number of reflections | 46773 | |
| <I/σ(I)> | 29.74 | 2.25 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 43 | 24.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 1UL PROTEIN PLUS 1UL RESERVOIR 0.1 M HEPES PH 7.5, 0.1 M NACL, 1.6 M (NH4)2SO4 USING THE HANGING-DROP VAPOR-DIFFUSION METHOD |
