4AM3
Crystal structure of C. crescentus PNPase bound to RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 93.640, 112.060, 236.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.870 - 3.000 |
| R-factor | 0.21216 |
| Rwork | 0.210 |
| R-free | 0.25391 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gme |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.822 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.130 | 0.520 |
| Number of reflections | 50090 | |
| <I/σ(I)> | 9 | 2.7 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 24% WT/V PEG 3350, 0.1 M BIS-TRIS PH 5.5, 0.1 M AMMONIUM ACETATE |
