4AM2
Bacterioferritin from Blastochloris viridis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 170.200, 170.200, 170.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 98.260 - 1.800 |
| R-factor | 0.1565 |
| Rwork | 0.155 |
| R-free | 0.19160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gvy |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.817 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.150 | 0.700 |
| Number of reflections | 35784 | |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 94.6 | 98.8 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 1UL PROTEIN PLUS 1UL RESERVOIR SOLUTION OF 0.1M HEPES PH7.5, 0.1M NACL, 1.6M (NH4)2SO4 USING THE HANGING- DROP VAPOR-DIFFUSION |
