4ALF
Pseudomonas fluorescens PhoX in complex with phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2012-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.099, 71.035, 228.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.140 - 1.250 |
| R-factor | 0.1343 |
| Rwork | 0.133 |
| R-free | 0.16432 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zwu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.463 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.200 | 1.310 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 258332 | |
| <I/σ(I)> | 6.3 | 2.2 |
| Completeness [%] | 83.6 | 74.3 |
| Redundancy | 2.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRIS HCL PH 8.0, 0.2 M LICL, REMARK 280 20% W/V PEG 6000. THE CRYSTALS WERE SOAKED WITH 100 UL PHOSPHATE BUFFERED SALINE FOR HALF AN HOUR |
