4ALD

HUMAN MUSCLE FRUCTOSE 1,6-BISPHOSPHATE ALDOLASE COMPLEXED WITH FRUCTOSE 1,6-BISPHOSPHATE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	287
Spacegroup name	P 64 2 2
Unit cell lengths	96.500, 96.500, 166.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.560 - 2.800
R-factor	0.226 *
Rwork	0.202
R-free	0.29900 *
Structure solution method	OTHER
RMSD bond length	0.028
RMSD bond angle	0.078
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	19.560
High resolution limit [Å]	2.800
Rmerge	0.070 *
Number of reflections	11099
<I/σ(I)>	31
Completeness [%]	93.7
Redundancy	4.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5		Millar, J.R., (1981) Phil. Trans. R. Soc. Lond. B, 293, 209. *