4ALA
Structure of Dengue virus DIII in complex with Fab 2H12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.590, 126.040, 52.990 |
| Unit cell angles | 90.00, 95.74, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.840 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.110 | 0.980 |
| Number of reflections | 44605 | |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 97.0 | 80 |
| Redundancy | 7.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% PEG 3350, 0.2M DI-HYDROGEN PHOSPHATE, pH 7.5 |
