4AL7
Crystal structure of the Csy4-minimal crRNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-29 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.740, 47.280, 87.930 |
| Unit cell angles | 90.00, 106.72, 90.00 |
Refinement procedure
| Resolution | 36.476 - 2.320 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4al5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.540 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.480 | 2.380 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 10773 | |
| <I/σ(I)> | 13.5 | 2.49 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 4.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 21% PEG4000, 180 MM SODIUM CITRATE PH 5.0, 100 MM MAGNESIUM CHLORIDE, 2 MM AMMONIUM METAVANADATE |
