4AL5
Crystal structure of the Csy4-crRNA product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-23 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.790, 47.800, 86.570 |
| Unit cell angles | 90.00, 109.69, 90.00 |
Refinement procedure
| Resolution | 81.508 - 2.000 |
| R-factor | 0.1857 |
| Rwork | 0.183 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xlk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.328 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.510 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.810 |
| Number of reflections | 15945 | |
| <I/σ(I)> | 15.58 | 2.06 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 6.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 22 % PEG4000, 120 MM SODIUM CITRATE PH 5.0, 50 MM MGCL2 |
