4AKR
Crystal Structure of the cytoplasmic actin capping protein Cap32_34 from Dictyostelium discoideum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 124.460, 124.460, 77.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1izn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.359 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.150 | 0.840 |
| Number of reflections | 60185 | |
| <I/σ(I)> | 14.15 | 3.83 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES PH 7.5, 17% (W/V) PEG 8000 |
