4AKQ
Mutations in the neighbourhood of CotA-laccase trinuclear site: E498D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.833, 101.833, 136.565 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.820 - 2.100 |
| R-factor | 0.17876 |
| Rwork | 0.177 |
| R-free | 0.20623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w6l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.070 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 48362 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 10% PEG 4K, 0.1 M SODIUM CITRATE PH 5.5, 26% ISOPROPANOL |
