4AKL
Structure of the Crimean-Congo Haemorrhagic Fever Virus Nucleocapsid Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-25 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.380, 72.060, 101.230 |
| Unit cell angles | 90.00, 110.70, 90.00 |
Refinement procedure
| Resolution | 94.700 - 2.100 |
| R-factor | 0.18639 |
| Rwork | 0.184 |
| R-free | 0.23359 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.559 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.470 |
| Number of reflections | 56441 | |
| <I/σ(I)> | 13.9 | 2.5 |
| Completeness [%] | 95.5 | 97.2 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M BIS-TRIS PROPANE PH 6.5, 0.2 M NACL, 25% PEG 600 |
