4AKH
Dynein Motor Domain - AMPPNP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 175.560, 118.130, 201.020 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.24412 |
| Rwork | 0.241 |
| R-free | 0.30215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ai6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.487 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.300 | 3.940 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.100 | 0.690 |
| Number of reflections | 94930 | |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 99.2 | 97.5 |
| Redundancy | 5.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
