4AKF

Crystal structure of VipD from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	93
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	I 4 3 2
Unit cell lengths	252.757, 252.757, 252.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.2144
Rwork	0.214
R-free	0.23330
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.327
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.090
Number of reflections	34134
<I/σ(I)>	18.5	2.3
Completeness [%]	99.4	98.9
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN WAS CRYSTALLIZED FROM 100 MM TRIS-HCL (PH 8.0), 1.0 M AMMONIUM CITRATE TRIBASIC (PH 7.0) AND 10 MM MGCL2.