4AK9

Structure of chloroplast FtsY from Physcomitrella patens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-27
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	67.580, 123.460, 75.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.672 - 1.800
R-factor	0.217
Rwork	0.215
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2og2
RMSD bond length	0.011
RMSD bond angle	1.250
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.140	0.940
Number of reflections	58928
<I/σ(I)>	15.89	4.28
Completeness [%]	100.0	100
Redundancy	21.4	26.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.6		0.2M SODIUM NITRATE, 0.1M BIS-TRIS PROPANE PH6.5, 20% PEG3350, pH 6.6