4AIX
Crystallographic structure of an amyloidogenic variant, 3rC34Y, of the germinal line lambda 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 31 |
| Unit cell lengths | 41.884, 41.884, 170.807 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.869 - 1.800 |
| R-factor | 0.1711 |
| Rwork | 0.168 |
| R-free | 0.21190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lil |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.133 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.650 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.320 |
| Number of reflections | 31131 | |
| <I/σ(I)> | 10.4 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3 | 2.3 M AMMONIUM SULFATE, 40 % TREHALOSE |
