4AIS
A complex structure of BtGH84
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-26 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.330, 93.770, 99.160 |
| Unit cell angles | 104.27, 94.01, 102.97 |
Refinement procedure
| Resolution | 33.770 - 2.000 |
| R-factor | 0.19594 |
| Rwork | 0.193 |
| R-free | 0.24406 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cho |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.633 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.380 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.410 |
| Number of reflections | 113272 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 96.8 | 95.8 |
| Redundancy | 2.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.3 M AMMONIUM ACETATE, 0.1 M MES PH 6.0, 15% PEG 3350(W/V), 10% GLYCEROL |
