4AIO
Crystal structure of the starch debranching enzyme barley limit dextrinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-21 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.058, 82.072, 59.378 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 33.694 - 1.900 |
| R-factor | 0.18871 |
| Rwork | 0.187 |
| R-free | 0.22476 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y4s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.540 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.040 |
| Number of reflections | 60352 | |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 91.3 | 94.5 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN STOCK: 10 MG/ML PROTEIN 50 MM MES PH 6.6, 250 MM NACL, 0.5 MM CACL2 AND 0.67 MM MALTOTRIOSE. RESERVOIR: 30% POLYETHYLENE GLYCOL (PEG) 3350, 5% GLYCEROL, AND 0.3 M NAI. CYSTEIN WAS ADDED TO THE DROPS TO A FINAL CONCENTRATION OF 5-7 MM |
