4AIL
Crystal Structure of an Evolved Replicating DNA Polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-20 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 92.850, 126.770, 205.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 102.820 - 2.900 |
| R-factor | 0.22873 |
| Rwork | 0.227 |
| R-free | 0.25933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ahc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.825 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.520 | 3.060 |
| High resolution limit [Å] | 1.900 | 2.900 |
| Rmerge | 0.080 | 0.730 |
| Number of reflections | 26919 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 98.8 | 98.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M SODIUM CACODYLATE PH 6.5, 0.1 M SODIUM CITRATE, 30 % ISOPROPANOL, 10 MM DCTP |
