4AIK
Crystal structure of RovA from Yersinia in complex with an invasin promoter fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 3 |
| Unit cell lengths | 88.678, 88.678, 67.503 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.810 - 1.850 |
| R-factor | 0.19988 |
| Rwork | 0.198 |
| R-free | 0.23025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3deu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 2.100 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.034 | 0.487 |
| Number of reflections | 49796 | 7414 |
| <I/σ(I)> | 13.9 | 2.5 |
| Completeness [%] | 98.2 | 99.9 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 10 MM MGCL2, 50 MM MES PH 5.6, 2.3 M LISO4 |
