4AIE

Structure of glucan-1,6-alpha-glucosidase from Lactobacillus acidophilus NCFM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-16
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	55.830, 107.250, 103.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.424 - 2.050
R-factor	0.1395
Rwork	0.137
R-free	0.18340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zic
RMSD bond length	0.010
RMSD bond angle	1.237
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.050	0.210
Number of reflections	39496
<I/σ(I)>	25.5	9.7
Completeness [%]	97.2	91.2
Redundancy	5.3	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20% GLYCEROL, 16% PEG 8K, 0.1 M MES PH 6.5