4AIB

Crystal Structure of Ornithine Decarboxylase from Entamoeba histolytica.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-01-15
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	76.671, 119.283, 179.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.500 - 2.870
R-factor	0.253
Rwork	0.253
R-free	0.29934
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2on3
RMSD bond length	0.005
RMSD bond angle	0.831
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.920
High resolution limit [Å]	2.870	2.870
Rmerge	0.150	0.540
Number of reflections	35570
<I/σ(I)>	4.82	2
Completeness [%]	92.1	59
Redundancy	3.4	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				POLYETHYLENE GLYCOL, LITHIUM CHLORIDE