4AHK
K54E - Angiogenin mutants and amyotrophic lateral sclerosis - a biochemical and biological analysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-13 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.396, 37.338, 83.237 |
Unit cell angles | 90.00, 119.92, 90.00 |
Refinement procedure
Resolution | 36.072 - 1.970 |
R-factor | 0.2171 |
Rwork | 0.214 |
R-free | 0.27910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ang |
RMSD bond length | 0.006 |
RMSD bond angle | 1.010 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.080 | 0.230 |
Number of reflections | 23796 | |
<I/σ(I)> | 7.3 | 2.9 |
Completeness [%] | 88.8 | 72.9 |
Redundancy | 2.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20 % PEG 4K, 0.4 M NA/K TARTRATE, 0.1 M NA CITRATE PH 5.5 |