4AHA
Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with fucosylated monosaccharides (Fuc1-2Gal, Fuc1- 3GlcNAc, Fuc1-4GlcNAc and Fuc1-6GlcNAc)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.730, 88.380, 78.580 |
| Unit cell angles | 90.00, 99.63, 90.00 |
Refinement procedure
| Resolution | 77.470 - 2.200 |
| R-factor | 0.16699 |
| Rwork | 0.165 |
| R-free | 0.21169 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4agi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.535 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.410 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.120 | 0.380 |
| Number of reflections | 27431 | |
| <I/σ(I)> | 7.1 | 2.7 |
| Completeness [%] | 87.6 | 71.8 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM CACL2, 25% PEG 4K AND 100 MM TRIS, PH 8.5 |
