4AGR
Structure of a tetrameric galectin from Cinachyrella sp. (Ball sponge)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.182, 117.137, 62.064 |
| Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
| Resolution | 29.880 - 2.100 |
| R-factor | 0.20619 |
| Rwork | 0.202 |
| R-free | 0.28081 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1is5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.944 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.040 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 31308 | |
| <I/σ(I)> | 17.06 | 2.97 |
| Completeness [%] | 91.9 | 70.9 |
| Redundancy | 4.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M AMMONIUM SULFATE, 0.1 M MES PH 6.5, 30% PEG 5000 MME |
