4AGA
Hofmeister effects of ionic liquids in protein crystallization: direct and water-mediated interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 63 |
| Detector technology | CCD |
| Collection date | 2011-11-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.350, 78.350, 36.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.170 - 1.500 |
| R-factor | 0.16971 |
| Rwork | 0.168 |
| R-free | 0.21094 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.161 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.170 | 1.510 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.600 |
| Number of reflections | 20915 | |
| <I/σ(I)> | 19.33 | 3.19 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.35 | 6.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | CHICKEN EGG WHITE LYSOZYME (FLUKA) HAS BEEN DISSOLVED IN 50 MM SODIUM ACETATE BUFFER PH 4.57 AND CRYSTALLIZED BY SITTING DROP DIFFUSION METHOD. EACH DROP CONTAINED 20 MG/ML OF LYSOZYME AND 5.5 PERCENT OF NACL IN 25 MM OF NA[AC] PH 4.57 |
