4AG0
Crystal structure of FimX EAL domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.530, 87.660, 108.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.626 - 2.304 |
| R-factor | 0.2127 |
| Rwork | 0.209 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r6o |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.551 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.050 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.430 |
| Number of reflections | 27564 | |
| <I/σ(I)> | 22.21 | 3.13 |
| Completeness [%] | 99.0 | 98.2 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M NA ACETATE PH 4.5, 0.4 M K2HPO4, 1.2 M NAH2PO4 |
