4AFY
Crystal structure of the FimX EAL domain in complex with reaction product pGpG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 104.330, 104.330, 154.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.209 - 2.010 |
| R-factor | 0.2101 |
| Rwork | 0.208 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r6o |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.407 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.060 | 0.380 |
| Number of reflections | 57232 | |
| <I/σ(I)> | 30.6 | 5.8 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 13.6 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | HEPES 0.1 M PH 8.0, NAH2PO4 0.8 M K2HPO4 0.8 M |
