4AFP
The structure of metacaspase 2 from T. brucei determined in the presence of Samarium
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-26 |
| Detector | ADSC CCD |
| Wavelength(s) | 1.84560, 1.805, 1.846 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.217, 49.787, 85.224 |
| Unit cell angles | 90.00, 117.10, 90.00 |
Refinement procedure
| Resolution | 75.870 - 2.100 |
| R-factor | 0.18405 |
| Rwork | 0.181 |
| R-free | 0.23787 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.204 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.290 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.090 |
| Number of reflections | 16074 | |
| <I/σ(I)> | 21.5 | 10.5 |
| Completeness [%] | 93.7 | 68.2 |
| Redundancy | 6.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 50 MM HEPES PH 7.0, 0.1% TRYPTONE, 20% PEG 3350 |
