4AFK
In meso structure of alginate transporter, AlgE, from Pseudomonas aeruginosa, PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-22 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.253, 74.416, 115.529 |
| Unit cell angles | 90.00, 101.56, 90.00 |
Refinement procedure
| Resolution | 19.770 - 1.897 |
| R-factor | 0.1656 |
| Rwork | 0.163 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rbh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.344 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.180 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.140 | 0.350 |
| Number of reflections | 37703 | |
| <I/σ(I)> | 15.7 | 2.9 |
| Completeness [%] | 100.0 | 84.9 |
| Redundancy | 6.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.5 | 0.1 M TRIS-HCL, PH 7.5, 0.1 M SODIUM CITRATE, 18 %(W/V) PEG 400. PROTEIN WAS MIXED WITH 7.8 MAG AT RATIO 1:1 (W:W) TO FORM CUBIC PHASE. |
