4AFI
Complex between Vamp7 longin domain and fragment of delta-adaptin from AP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-28 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.555, 63.555, 217.915 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.010 - 2.800 |
| R-factor | 0.23164 |
| Rwork | 0.231 |
| R-free | 0.25266 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vx8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.566 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.310 | 2.900 |
| Number of reflections | 11810 | |
| <I/σ(I)> | 7.3 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 4 M NACL, 100 MM BICINE PH 9.0, 10 MM PRASEODYMIUM ACETATE |
