4AFF
High resolution structure of a PII mutant (I86N) protein in complex with ATP, MG and FLC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 62.087, 62.087, 51.116 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 137.000 - 1.050 |
| R-factor | 0.13 |
| Rwork | 0.120 |
| R-free | 0.14000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.428 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.090 | 0.740 |
| Number of reflections | 49584 | |
| <I/σ(I)> | 12.2 | 2.1 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 7.2 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | pH 5.6 |
