4AF6
PEA FNR L268V MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.975, 94.698, 138.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.24413 |
| Rwork | 0.241 |
| R-free | 0.28415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qg0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.600 | 3.060 |
| High resolution limit [Å] | 2.200 | 2.900 |
| Rmerge | 0.120 | 0.600 |
| Number of reflections | 22283 | |
| <I/σ(I)> | 11 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% PEG 6000, 5% 2-METHYL-2, 4-PENTANEDIOL, 0.1M HEPES PH 7.5 |
