4AEQ
Crystal structure of the dimeric immunity protein Cmi solved by direct methods (Arcimboldo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.058, 83.552, 38.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.224 - 1.892 |
| R-factor | 0.2072 |
| Rwork | 0.206 |
| R-free | 0.22280 |
| Structure solution method | DIRECT METHODS |
| Starting model (for MR) | ALPHA HELIX |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.878 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.080 | 0.510 |
| Number of reflections | 15884 | |
| <I/σ(I)> | 11 | 2.5 |
| Completeness [%] | 96.6 | 90 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM SULFATE, 30% PEG 4000 |
