4AEF
THE CRYSTAL STRUCTURE OF THERMOSTABLE AMYLASE FROM THE PYROCOCCUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 95 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 41 |
Unit cell lengths | 150.065, 150.065, 67.856 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.430 - 2.340 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j0h |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.430 | 2.420 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.070 | 0.250 |
Number of reflections | 61332 | |
<I/σ(I)> | 22.9 | 5.87 |
Completeness [%] | 95.8 | 98.6 |
Redundancy | 5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 2% POLYETHYLENE GLYCOL (PEG)400, 2M AMMONIUM SULFATE, 0.1M HEPES BUFFER PH 7.5 |