4AE8
Crystal structure of human THEM4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.539, 58.211, 69.681 |
| Unit cell angles | 89.96, 71.14, 64.60 |
Refinement procedure
| Resolution | 29.720 - 1.594 |
| R-factor | 0.1867 |
| Rwork | 0.185 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.023 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.220 |
| Number of reflections | 83501 | |
| <I/σ(I)> | 22.7 | 3.2 |
| Completeness [%] | 93.9 | 84 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG3000, 0.2M NACL, 0.1M TRIS PH 7.0 |
