4ADJ
Crystal structure of the Rubella virus glycoprotein E1 in its post-fusion form crystallized in presence of 1mM of calcium acetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2010-09-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 121.875, 126.899, 130.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.800 - 1.940 |
| R-factor | 0.1825 |
| Rwork | 0.182 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4adi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 2.080 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 145923 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 97.1 | 81.5 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.4% PEG 8K, 100MM NAHEPES PH 8, 25% GLYCEROL, 1MM CAOACT |
