4ADG

Crystal structure of the Rubella virus envelope Glycoprotein E1 in post-fusion form (crystal form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	110
Detector technology	PIXEL
Collection date	2008-05-23
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	121.680, 126.550, 130.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.180
R-factor	0.1786
Rwork	0.178
R-free	0.19340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ad1
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.340	2.300
High resolution limit [Å]	2.180	2.180
Rmerge	0.110	0.500
Number of reflections	104002
<I/σ(I)>	19.5	3.4
Completeness [%]	99.1	97.4
Redundancy	12.4	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				2-3% PEG4000, 100MM HEPES PH 7.5-8.0, 30% GLYCEROL. E1 PROTEIN WAS SOAKED WITH 25MM OF GALACTOSE 3-SULFATE (GAL3S, HEAD GROUP OF SULFATIDE) DURING ONE WEEK.