4ADG
Crystal structure of the Rubella virus envelope Glycoprotein E1 in post-fusion form (crystal form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2008-05-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 121.680, 126.550, 130.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.180 |
| R-factor | 0.1786 |
| Rwork | 0.178 |
| R-free | 0.19340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ad1 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.340 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.110 | 0.500 |
| Number of reflections | 104002 | |
| <I/σ(I)> | 19.5 | 3.4 |
| Completeness [%] | 99.1 | 97.4 |
| Redundancy | 12.4 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2-3% PEG4000, 100MM HEPES PH 7.5-8.0, 30% GLYCEROL. E1 PROTEIN WAS SOAKED WITH 25MM OF GALACTOSE 3-SULFATE (GAL3S, HEAD GROUP OF SULFATIDE) DURING ONE WEEK. |
